Sania Mirza failed to make the singles main draw of the WTA Aegon International after losing in the third round of the qualifying event to Russia's Ekaterina Makarova in Eastbourne on Monday.
The eighth seeded Indian was beaten 4-6, 3-6 in 68 minutes by her unseeded opponent in the final qualifying round match of the grass court event.
Sania saved six break-points in the first set but one break of serve proved costly as he conceded the lead.
In the second set she was successful in grabbing the chance to break, but also dropped serve twice in the process and bowed out.