Uruguayan Andres Cunha will referee the World Cup semi-final between France and Belgium at the St Petersburg Stadium on Tuesday evening, FIFA said on Monday.
Cunha has already officiated at two group stage matches at the tournament - France's victory over Australia and Spain's defeat of Iran.
The 41-year-old gave France a penalty in the 2-1 win over Australia, the first time a spot kick had been awarded at a World Cup after reference to the Video Assistant Referee (VAR).
Cunha, who will be assisted on the touchline by compatriots Nicolas Taran and Mauricio Espinoza, booked three Australians, two Iranians and one Frenchman over his first two matches.
Croatia's Football Federation sacks Vukojevic over Ukraine message
Croatia's Football Federation (HNS) sacked Ognjen Vukojevic, a member of the national team's coaching staff, over a "glory to Ukraine" video posted after Croatia beat Russia in the World Cup quarter-finals, HNS said in a statement on Monday.
Vukojevic and Croatia defender Domagoj Vida published the video on social media after the match on Saturday, which was criticised by Russian politicians and led to a warning from FIFA's disciplinary committee.
Relations between Ukraine and World Cup hosts Russia remain fraught after the latter's 2014 annexation of the Crimean peninsula and its backing for a pro-Russian uprising in the east of the country.
HNS said it has revoked Vukojevic's team accreditation at the World Cup and relieved him of his duties as an observer for the Croatia squad.
"HNS hereby apologises to the Russian public for the actions of a member of the Croatian delegation," it said.
It said that Vukojevic and Vida also apologised for their statements, saying they were not intended to be political messages but "unfortunately left room for such interpretations".
Both Vukojevic and the 29-year-old Vida previously played for Ukrainian club Dynamo Kiev.
Croatia face England in the semi-finals of the tournament in Moscow on Wednesday.
FIFA WC: All you must know about Belgium-France semis history
With varied heritage, Belgians keep African interest alive at World Cup
FIFA World Cup: French icon Henry plots his country's downfall
Brazil fans greet team after World Cup knockout
FIFA World Cup: 42 per cent of goals have come from set-pieces