Courtois was sent off for denying a goal scoring opportunity in the 52nd minute of Chelsea's 2-2 draw against Swansea City at Stamford Bridge on Saturday.
An independent regulatory commission rejected Chelsea's appeal against the red card and upheld a one-match suspension, the FA said.
The ruling will give back-up keeper Asmir Begovic, signed from Stoke City earlier this summer as a replacement for Petr Cech, the chance to make his first Premier League start for Chelsea.
Image: Thibaut Courtois
Photograph: Julian Finney/Getty Images
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