- Beijing Olympics: Top 10 moments
The 22-year-old Jamaican, who set world records in winning the 100 metres, 200 metres and 4x100 metres relay in Beijing, finished Friday's race in 9.83 seconds, 0.16 seconds clear of America's double Olympic bronze medallist Walter Dix.
After taking silver behind Bolt at the Olympics, Trinidad & Tobago's Richard Thompson had to settle for third place in Zurich, a further 10th of a second behind Dix.
The only signs of sluggishness by Bolt came at the start of the race with official reaction times showing him to be the slowest starter of the nine runners, though his now famous lengthy strides were more than enough to compensate.
"It was good, but my start was not too good," said Bolt. "I concentrated on winning and as I'm starting to get a cold I was not able to think about any faster time."
Fellow Olympic champion and world record holder Dayron Robles was also able to back up his stunning 110 metres hurdles performance in Beijing, but had to work a good deal harder for his latest victory than Bolt.
The 21-year-old Cuban ran neck and neck with Olympic bronze medal winner David Oliver before ducking his head at the line to finish in 12.97 seconds, 0.01 seconds ahead of the American.
Kenyan teenager Pamela Jelimo continued her fantastic season, following up her Olympic gold medal in the 800 metres by setting the year's best time of one minute 54.01 seconds.
Jelimo, 18, also kept herself well on track for a share of the Golden League's $1 million jackpot awarded to athletes who win their event at all six of the season's meetings.
With one meeting left in Brussels, Jelimo faces competition for the main prize only from Croatian high jumper Blanka Vlasic.
Photograph: Getty Images | Text: Reuters