Real Madrid forward Karim Benzema has not recovered sufficiently from a knee injury and has been left out of the squad for Tuesday's Champions League semi-final first leg at Juventus, the holders said on Monday.
Real's Mexico forward Javier Hernandez could start upfront in Turin alongside Cristiano Ronaldo and Gareth Bale, who returned from a calf problem as a second-half substitute in Saturday's 3-2 La Liga win at Sevilla.
Another option would be for Ancelotti to switch to a 4-4-2 formation, with Hernandez dropping to the bench and Isco coming into the midfield alongside Toni Kroos, James Rodriguez and converted centre back Sergio Ramos.
Real are seeking a record-extending 11th European crown, while Italian champions Juve are chasing their third.
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