Shops, business establishments, banks, financial and educational institutions remained closed while traffic was thin on the national highways, official sources said in Guwahati.
Barring a few government-owned buses, there was no traffic on roads but trains and flights plied as scheduled.
Security measures have been tightened as all major entry and exit routes of important cities and towns were sealed.
Additional police pickets have been posted atop the Neelachal hills where the Ambubachi mela of Kamakhya temple was on with lakhs of devotees thronging the temple.
The ULFA called the bandh to protest against heightened operation by security forces against it and other militant outfits in what it termed as fake encounters.
Nearly 30 ultras, including six of ULFA and 24 others belonging to the National Democratic Front of Bodoland have been killed in operation by security forces in the last one month in the state.