Addressing a large gathering at the Gaddafi Stadium, the Lashkar-e-Tayiba founder called for unity among Muslim community "to fail the design of infidels".
He said international players are conspiring against Pakistan's nuclear programme.
"The US India are signing agreements against Pakistan and there has been a preparation of a big war", he said, alleging "drones are parked at Indian airports and our rulers are engaged in a fight with opposition."
Saeed, on whose head the US has put a bounty of $10 million, termed Pakistan and Saudi Arabia's growing distance with the US as a "blessing in disguise".
"It is a blessing in disguise that Pakistan and Saudi Arabia are getting distanced from America. This will lay foundation of the Islamic Union which is the need of the hour for the Islamic world," he said.
He condemned the terror attack in Saudi Arabia and alleged the involvement of "foreign hands" in destabilising the Muslim world.
A large number of people offered their prayer behind Saeed amid tight security with police personnel and JuD security wing members deployed in and outside the stadium.
Saeed has been leading the Eid prayers at Gaddafi Stadium for the last many years.
India has repeatedly asked Pakistan to bring Saeed to justice for masterminding the 2008 Mumbai attacks. He has often been seen addressing anti-India rallies in Pakistan. Islamabad says it has no proof against the JuD chief.
A total of 166 people were killed in the attack orchestrated by the LeT in November 2008.