The BSP supremo, along with her party colleagues, came into House and started speaking even before taking her seat.
As the chairman tried to pacify Mayawati and asked her to allow the Question Hour to proceed, the BSP chief went into the Well with her party members continuing with her verbal attack.
Mayawati's remarks came on a day when the Chairman has called a meeting of the rules committee of the Rajya Sabha anguished over frequent disruptions of the Question Hour.
Mayawati's party colleagues started shouting 'Dalit virodhi ye sarkar nahi chalegi' (anti-Dalit government is not acceptable).
A visibly upset Ansari adjourned the House till noon.
When the House reassembled, BSP members again trooped into the Well shouting anti-government slogans.
On Monday, Mayawati had threatened to take a "tough stand" in case the government does not help bring order in the Rajya Sabha and get the bill passed.
Amid the uproar, Bharatiya Janata Party leader M Venkaiah Naidu took strong objection to reports on the government's plans to increase the cap on subsidised LPG.
As the din continued, Deputy Chairman P J Kurien adjourned the House for another 30 minutes till 12.30 pm. However, there was no improvement in the situation when the House met again and minutes after it was adjourned for the day.
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