Two of them, P M Mannan and S Ezhilmannan, are the party's executive and general council members.
DMK general secretary K Anbazhagan said the high command had received information that despite party president M Karunanidhi's warning, once again "mischievous posters" had come up in Madurai. He did not elaborate on the posters' content.
"Mannan, Ezhilmannan, Mubarak Mandiri, Anbarasu Elango and M Balaji are suspended from all posts, including primary membership of the party since they were repeatedly engaged in activities bringing disrepute to the party despite Kalaignar's (Karunanidhi) warning," Anbazhagan said in a statement.
Amidst sibling rivalry between Karunanidhi's sons -- Alagiri and M K Stalin -- over who is the political heir apparent to their father, DMK high command had on Saturday dissolved its Madurai Urban unit, replacing it with an ad-hoc body dominated by supporters of Stalin.
The party acted in the wake of a controversial poster being pasted by supporters of Alagiri, making critical remarks in the context of DMK's general council held last month in Chennai, which the former Union minister did not attend.
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