K Chandrasekhar Rao-led TRS has 17 MLAs in 294-member House and is banking on the support of Communist Party of India, Bharatiya Janata Party, Lok Satta Party and some Independents.
To a query if the party has the backing of 30 MLAs, the required minimum number needed to introduce the no-confidence motion, TRS MLA T Harish Rao replied in the affirmative.
Separately, YSRCP, which has 17 MLAs, also submitted its notice to the Speaker.
YSRCP MLA Shobha Nagireddy said, "Her party is ready to extend support to whoever moves the no-confidence motion on people's problems".
Meanwhile, the Assembly was adjourned for an hour, minutes after the session began as Opposition members trooped into the well of the House and raised slogans.
Andhra: TRS set to move no-trust motion against Cong govt
View: Why the Italians take India for granted
Rahul gets more than he bargained for at Cong meeting
Consultation on separate Telangana is on: Congress
TRS loses face in its own backyard