The video, which may have been filmed in Afghanistan, has images of bin Laden along with senior Al Qaeda members Ramzi bin al-Shibh and Abu Hafs al Masri.
The footage is rare since bin Laden has never before been seen with al-Shibh, who was captured almost three years ago to the day in Karachi after a gunbattle.
Al Jazeera said two men seen with bin Laden, al-Shibh and al Masri were Wael al Shahri and Hamza al Ghamedi.
Al Shahri was one of the hijackers on the American Airlines flight which crashed into the World Trade Centre's south tower. Al Ghamedi was a hijacker on the United Airlines flight which crashed into the WTC's north tower 17 minutes later on that terrible Tuesday.
Both men are seen recording their wills on the video, after which bin Laden hugs them.
Meanwhile, the US government revealed on Thursday that bin al-Shibh and Khalid Sheikh Mohammad, who has been called the real mastermind behind the 9/11 attacks, also planned to attack different targets in London in 2003.
Mohammad was captured in Pakistan in 2003 and the plan was busted soon after.
The new information is apparently the outcome of the interrogation of bin al-Shibh and Mohammad after their arrests in 2002 and 2003 respectively, which the Americans have made public for the first time.