The court rejected Ahuja's bail on the ground that it is a 'serious offence' and there is prima facie evidence against him.
The court had rejected his bail plea on July 8 on the same grounds.
The 36-year-old actor again filed for bail on September 5 after the city police filed its chargesheet against him.
Charges against Ahuja, who is currently lodged in Mumbai's Arthur Road jail, will be framed on September 23.
His lawyer Shrikant Shivade said he would challenge the lower court's order in the Bombay high court.
The actor's case was transferred to a fast track court after Maharashtra Chief Minister Ashok Chavan gave an assurance that the victim would get justice soon.