The MNS supporters stormed into the PVR theatre, in the Western suburb of Juhu in Mumbai, and tore down the posters of The Last Lear and broke the glass panes.
The move came after Jaya apologised for her irresponsible statement to a newspaper, and Amitabh claimed that he had a sleepless night.
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The premiere was to be held on Wednesday night, and in the presence of the cast including Amitabh Bachchan.
The Last Lear also stars Arjun Rampal and Preity Zinta, and is directed by Rituparno Ghosh. According to Ghosh, the film will still be released on the scheduled date of September 12.
Photograph: Arun Patil