Opening the batting, he adopted a cautious approach while facing 132 deliveries and hitting two boundaries, at St Lawrence ground.
He was dismissed by right-arm pacer Charlie Shreck in the 42nd over of the day, caught by Brendan Nash.
Since commencing his stint with Essex last month Gambhir had scores of 31, 21, 2 and 0 before registering a hundred on August 30 against Gloucestershire in a drawn match in Bristol.
The left-handed opener, however, had to return home on September 1 following a bereavement in the family.
Gambhir has not played for India since the home ODI series against England in January. He was later ruled out of the subsequent Test series against Australia due to jaundice after being selected for the first Test.
Image: Gautam Gambhir
Photograph: Getty Images
'Tendulkar still has hunger for cricket'
I am mentally stronger now and worked on my shot selection: Dhawan
Dravid backs Sehwag, Zaheer and Gambhir to return to Indian team
Sehwag, Gambhir, Zaheer get lifeline against West Indies 'A'
Gautam Gambhir to rejoin Essex today